1- write your hypothesis for the effect of increasing substrate concentration on
ID: 56058 • Letter: 1
Question
1- write your hypothesis for the effect of increasing substrate concentration on amylase activity.
2- You collected data in the form of time to starch disappearance. However, enzyme activity is usually quoted as a rate. Convert your collected data to rate form (mg of substrate consumed / unit time), and produce a graph of this data (rate vs. substrate concentration): hint: you must first convert the starch volumes to amounts using the C1V1 = C2V2 equation, and convert 1% starch in to lg/ 100mL equivalent,
Use Appendix 1 for hints using EXCEL
Which quantity, rate or substrate concentration, should be on which axis?
What trend does your data show, and does this support or fail to support the hypothesis you propose in #1, above?
3- What form of inhibition did you observe with PTU on catechol oxidase? Justify your position with a brief explanation
4- Many pharmaceutical agents act as inhibitors of enzymes in the body. Research any pharmaceutical agent you choose, and provide a reference(s) for the following:
The name of the drug
The enzyme it inhibits
The mechanism of its inhibition (competitive or non-competitive)
Tube # 1% Strach pH 6.8 buffer Distilled water 0.1% amylase Time to starch disappearance 1 0 mL 2 mL 2 mL 1 mL 30 2 0.5 mL 2 mL 1.5 mL 1 mL 300 3 1.0 mL 2 mL 1.0 mL 1 mL 360 4 1.5 mL 2 mL 0.5 mL 1 mL 8:03 5 2.0 mL 2 mL 0 mL 1 mL 11Explanation / Answer
4. Name of drug: Aciphex
Enzyme it inhibits: Proton pump
Mechanism: It is prescribed during gastric ulcers and other related disorders. It inhibits activity of H+/K+ ATPase pump which further prevents the secretion of gastric juice into the stomach.
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