The following DNA sequence contains an origin of replication, which has been cir

Question

The following DNA sequence contains an origin of replication, which has been circled. Given this sequence, circle the primer below that can be used to synthesize a leading strand from this origin. 5'- UCUAAG -3' 5'- AGATTC -3' 5'- TCTAAG -3' 5'- AGAUUC -3' Write the first 5 bases that would be added to this primer by DNA polymerase as the leading strand grows in order of synthesis. Be sure to label its 5' and 3' ends. After a nuclease removes this primer, write the sequence that a DNA polymerase could use to fill in the gap. Be sure to label its 5' and 3' ends. Is replication of this sequence now completed? Explain why or why not.

Explanation / Answer

Based on the given data,

a)

5’-AGATTC-3’

b)

GACGG

c)

5’AGATTC-3’

d)

Yes, in leading strand only primer sequence is enough to carry out entire stand synthesis. So, after nick sealing the DNA replication of this sequence is completed.    

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