One strand of a section of DNA isolated from E. coli reads: 5’-GTAGCCTACCCATAGG-
ID: 56250 • Letter: O
Question
One strand of a section of DNA isolated from E. coli reads:
5’-GTAGCCTACCCATAGG-3’
Suppose that an mRNA were transcribed using the complement of this DNA strand as the template. What would the sequence of the mRNA in this region be?
How many different peptides could potentially be made from this sequence of RNA? Would the same peptides be made if the other strand of the DNA served as the template for transcription?
What peptide would be made if translation started exactly at the 5’ end of the mRNA in part A? When rRNAAla leaves the ribosome, what tRNA will be bound next? When the amino group of alanine forms a peptide bond, what bonds, if any, are broken, and what happens to tRNAAla?
Explanation / Answer
mRNA transcribed using the complement of this DNA strand will be
5'-GUAGCCUACCCAUAGG-3'
This sequence of peptide can make three peptide.
Same peptide can be made if the other strand of the DNA serve as the template for transcription.
5' GUA - GCC - UAC - CCA- UAG
The next tRNA after ala is Tyr and the peptide sequence will be Ala- Tyr-leu-tyr.
The incoming tyr will bind with ala to form peptide bond. The tRNA ala will leave the site once the peptide bond is formed.
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