7. The enzyme mannose-6-p isomerase catalyzes the reversible reaction: Mannose-6

Question

7. The enzyme mannose-6-p isomerase catalyzes the reversible reaction: Mannose-6-P (M6P) Fructose-6-P (F6P). The fructose 6-P (FEP) formed can then enter glycolysis. The K 3.1 A) Calculate G for the reaction above at 25°C(Us Simt cant tutus). What does your answer tell you regarding spontaneity?(3g s) It Will proced Povwand wih a Ptv it be exottuvmic spon tauwou B) Assume you incubate 30 mi of a solution containing 0.075 M M6P overnight at 25'C with an excess of the enzyme mannose-6-P isomerase. How many millimoles of F6P will be in the 30 ml solution the next morning? (5 pts) C) Experimental values for the actual steady-state concentrations of these compounds in the cell are [M6PJ . 3 uM and [F6P1-14 M. Calculate &G;' at 25*C.(Use 2 significant figures). In which direction will the reaction proceed to reach equilibrium? Explain.(5 pts)

Explanation / Answer

g) If a solvent containing protons is used for NMR technique, then the spectrum thus obtained will be dominated by the solvent signal as solvent is always in excess, so it may interact with the protons of the analyte and give noise signals.

Although,deuteration is never cent per cent so signals for residual protons of undeuterated solvent may exist in the spectrum.For CDCl3, the residual CHCl3 signal can be seen at 7.26 ppm

h)

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