Using the table below, and the three bulleted points below. Calculate the net yi
ID: 56368 • Letter: U
Question
Using the table below, and the three bulleted points below. Calculate the net yield of ATP (assuming 4H+ are required for the synthesis of 1 ATP via ATP Synthase) from the complete oxidation of C14:0. Indicate the number of ATP (generated through substrate-level phosphorylation), Acetyl CoA, NADH, and FADH2 that are generated through this process.The net production should account for the “activation” of fatty acid by Acyl CoA Synthetase (equivalent to 2ATP).
•Total H+ produced through complete oxidation of C14:0 (from table below):
•Total Possible ATP based on number of H+ produced:
•Net ATP Produced from complete oxidation of C14:0
Total Number of Product
Molecules Produced
Total Number of H+ Produced in Electron Transport Chain
Atp
(from substrate-level phosphorylation in TCA cyle)
ProductTotal Number of Product
Molecules Produced
Total Number of H+ Produced in Electron Transport Chain
List Enzyme(s) Responsible for Product Generation Subcellular LocalizationAtp
(from substrate-level phosphorylation in TCA cyle)
Not applicable Acetyl CoA (from Beta-oxidation) Not applicable NADH ( from Beta-oxidation) NADH ( from TCA Cycle) FADH2 (from Beta-oxidation) FADH2 (from TCA Cycle)Explanation / Answer
Fatty acids undergo - oxidation i.e. oxidative removal of successive two-carbon units in the form of acetyl CoA. Thus the conversion of C 14:0 will yield 7 acetyl CoA (six passes of - oxidation occurs here).
Each pass through - oxidation gives one molecule of acetyl CoA, two pairs of electrons, and four protons.
Overall equation :
C14 :0 ------------à 7 acetyl CoA + 6FADH2 + 6NADH
Each molecule of FADH2 gives 1.5 molecule of ATP.
Each molecule of NADH gives 2.5 molecule of ATP.
Each acetyl CoA yields 1GTP (=ATP), 3 NADH,1FADH2
In total we have,
13 FADH2 + 27 NADH + 7 GTP (=ATP) = 94 ATP
Energetic cost of activating a fatty acid = 2 ATP
Hence net gain of ATP = 92 ATP
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