Q) Why do we run (electrophorese) our Native PAGE gel with mushroom extract at p
ID: 56452 • Letter: Q
Question
Q) Why do we run (electrophorese) our Native PAGE gel with mushroom extract at pH 8.3, but detect the enzyme at a different pH?
a) Alkaline pH gives most proteins at a net positive charge to cause migration towards the cathode during electrophoresis, but the enzyme is active at a different pH
b) For tyrosinase, it is actually better to perform electrophoresis and detection at the same pH
c) Alkaline pH gives most proteins at net negative charge to cause migration towards the anode during electrophoresis, but the enzyme is active at a different pH
d) Native PAGE seperates proteins based only on size, so th pH does not really matter
2) Cellular extracts are kept on ice in order to:
a) Prevent hydrolysis by enzymes releasedby breaking the cells
b) Slow any enzyme activities that are present
c) Preserve the structure of the proteins and other macromolecules
d) Preserve the activities of the proteins and other macromolecules
e) All of the above
3) All of the ATP made during glycolysis and th Citric Acide cycle is made by:
a) Electron transport
b) Oxidation reactions
c) Substrate level phosphorylation
d) Oxidative phosphorylation
e) Photophosphorylation
f) Chemiosmosis
4) Most of the energy of the glucose molecule is contained in its:
a) Hydroxyl group
b) Phosphates
c) Aldehyde group
d) Carbons
e) electrons
f) Protons
Explanation / Answer
1.
c) Alkaline pH gives most proteins at net negative charge to cause migration towards the anode during electrophoresis, but the enzyme is active at a different pH
At acidic pH most proteinsare positively charged
2.
b) Slow any enzyme activities that are present
The ice water preserve the Deoxyribonucleic acid by slowing down the enzymes which can break it away.
3
d) Oxidative phosphorylation
A process in which ATP is produced
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