Name number To complete the test get a rack of test tubes from the counter or yo
ID: 565641 • Letter: N
Question
Name number To complete the test get a rack of test tubes from the counter or your table top. They are labeled 1-7. You will have a bottle of laKl and a well plate. Each of the seven tubes have been filled with different ingredients. Review Table 1 to determine the differences and similarities between the contents of the tubes. You will remove a drop from each tube and place it in the well plate. You will then add I2Kl and determine if starch has been formed. Record the re was positive and a--in the box indicating the test was negative sults in Table 1 placing a+ in the box indicating the test Table 1: The numbered tubes with their ingredients. Ingredient Tube 1 Tube 2 Tube 3 Tube 4 Tube 5 Tube 6 Tube 7 Water 1 ml 1 ml1 4 ml 3 ml 1 ml glucose 3 ml PH10 buffer pH 7 buffer x Glucose-1-x phosphate Enzyme 3 ml Boiled 1 ml 3 ml x 3 ml 3 ml 3 ml 3 ml 3 ml x 3 ml x enzyme Results pH 10 is an extreme buffer pH 7 is a neutral buffer KHaPO4 (potassium phosphate) is an alternative phosphate source Glucose is a sugar sourceExplanation / Answer
1. I2KI test will not work on Lipids and proteins. it works for the presence of Starch.
2. Starch is not formed in Test tube-1 because enzymes are not present which can convert glucose to starch.
3. enzymes are specific in action and structure. In test Tube-2 glucose and phospahte are separate. so no impact of enzyme. therefore it works on Glucose-1-phosphate.
4. It gave negative result in Test tube-4 because enzymes get denaturised when boiled due to high temperature.
5. In test tube -5 Buffer with pH 10 is their . Due to high pH value no reaction tyakes place to give proper result.
6. Due to absence of enzyme no reaction occured.
7. reaction occured in Test tube 3 &6 because it had enzyme present in both the test tubes, which act as biological catalyst and bring about required results.
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