1) With how many bacteria should you inoculate a broth if you wish to obtain 327
ID: 56583 • Letter: 1
Question
1) With how many bacteria should you inoculate a broth if you wish to obtain 32768 bacteria after 24 hours? Assume that the bacteria double every 3 hours?
2)There are initially 2 E.coli bacteria in 200 grams of hamburger meat. Health Canada considers that a load equal to or higher than 20 bacteria per 100 grams of meat represents a risk for the health of consumers. If the growth rate of E.coli at 4 degree C(mean temperature of a refrigerator) is 0.01 cell/min. What would be the maximum amount of time that the meat could be stored in the fridge before it is non comestible?
Explanation / Answer
1) With how many bacteria should you inoculate a broth if you wish to obtain 32768 bacteria after 24 hours? Assume that the bacteria double every 3 hours?
let the number of bacteria should you inoculate a broth be x
as bacteria doubles every 3 hours
y=x*(2)t/3
where x is initial amount ,y =amount after t hours
after 24 hours
32768=x*(2)24/3
32768=x*(2)8
x=32768/256
x=128
128 bacteria you should inoculate a broth if you wish to obtain 32768 bacteria after 24 hours
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