Thin Layer Chromatography (TIC) POST LAB QUESTINS NMEnn biShmort NAMr.Mychm feb.
ID: 567585 • Letter: T
Question
Thin Layer Chromatography (TIC) POST LAB QUESTINS NMEnn biShmort NAMr.Mychm feb. a,2018 1) List the applications of TLC? 2) What is the mobile phase in TLC? 3) What is the Stationary phase in TLC? 4) You are trying to determine a TLC solvent system which will separate the compounds x, Y, and 2. You ran the compounds on a TLC plate using hexanes/ethyl acetate 95:5 as the eluting solvent and obtained the chromatogram below. How could you change the solvent system to give better separation of these three compounds? 5) After a rather lengthy organic chemistry synthesis procedure, a student ran the product of the reaction on a TLC plate and obtained the result below. What might he/she have done wrong,if anything?Explanation / Answer
1. TLC is used for the sepation of organic compounds. The three industrial applications for TLC are in clinical, pharmaceutical, and food testing. The clinical tests performed with TLC is for the presence of drugs of abuse. In the pharmaceutical industry, TLC is used in production and quality assurance applications. In the food industry, TLC is used for many different applications, ranging from lipid separation to food dye analysis.
2. The mobile phase in TLC is the solvent system used to run the solute in the TLC. eg ethyl acetate, hexane,
DCM, methanol etc.
3. The stationary phase is the compound from wcich TLC is made of eg silica, alumina etc.
4. In Hexane : ethyl acetate (95:5) system there is no separation. To see better separation we can eithe reduce the ethyl acetate ratio or we can use less polar solvent like dichloromethane instead of ethyl acetate.
5 The student has sppoted the cancentrated spot which drags in the solvent system and could not see better separation. The student should spot dilute sapmle
Related Questions
drjack9650@gmail.com
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.