BI0370 Homework Set #21 Kinetics Spring 2018 All number answers with units must
ID: 568480 • Letter: B
Question
BI0370 Homework Set #21 Kinetics Spring 2018 All number answers with units must include them. Work must be shown for full credit 1. Consider the following three enzymes: a. Which has the highest affinity for substrate? How doEnzyme you know (briefly)? Kar (sec) 5x10s Sx10 5x102 2.5x10 b. Which converts the most substrate to product in a given time period? How do you know (briefly)? What is the catalytic efficiency of each enzyme? Which is most efficient? How do you know? Explain the efficiency of each enzyme. c.Explanation / Answer
a)
the enzyme B will have highest efficiency for substrate .
because it's concentration is highest of all other enzymes.
b)
the enzyme B will convert much more substrate into product in a given time,
because the concentration of other enzymes is low so they might get saturated.
c)
the catalytic efficiency will be K cat/ K m
Enzyme A= 2.5×(10)4/5×(10)5
= 0.05
Enzyme B= 250/5×(10)7
= 0.000005
Enzyme C= 2.5×(10)7/ 5×(10)2
= 5× (10)4
The enzyme c will be having highest catalytic efficiency.
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