Academic Integrity: tutoring, explanations, and feedback — we don’t complete graded work or submit on a student’s behalf.

(3 points) Which of the following is LESS likely to represent a chromatogram of

ID: 569480 • Letter: #

Question

(3 points) Which of the following is LESS likely to represent a chromatogram of a mixture with two components? Circleyourchoice clearly andg eyourreasoning briefly, 7. 91o 11 12 13 14 15 36 17 18 19 20 21 22 31234567 9 1011 12 13 14 15 61718 19 20 21 22 23 Give the reasoning behind your choice (10 points)Suppose you had two peaks (similar to those in the diagram above in #7) that have poor resolution Rs. 8" a) Which of the following could help resolve the peaks? Give your reason Would the effect be better Decrease the sample injection time Adjust flow rate to increase H, the theoretical plate height Adjust flow rate to decrease H, the theoretical plate height b) Describe one additional way to increase resolution using a Gc. c) Describe one additional to increase resolution using an HPLC.

Explanation / Answer

7. The width of a peak at half of the peak height is directly proportional to the retention time. Therefore, it is expected that, broader peak will have higher retention time. The chromatogram on the left is correct. The chromatogram on the right (with sharp peak at higher retention time) is unexpected and less likely to represent a mixture of two components.