How does spectroscopy relate to light? o What are different types of light o How

Question

How does spectroscopy relate to light? o What are different types of light o How is spectroscopy used for everyday life How did your results show the relationship between transmittance and absorbance? Back Discuss the relationship between concentration and absorbance up with your results as evidence o Did your results back this up? Explain o What happened as each solutions' concentration got higher? o How did you use that relationship to determine your unknowns' concentration? . In the prelab reading and assignment, you were asked to look into the Beer Lambert Law In your own words, discuss this law and how it relates to this experiment.

Explanation / Answer

1) Spectroscopic techniques are mainly used to irradiate a particular molecule with a particular frequency of light(or wave) to see how much energy it is absorbing(Absorption Spectra) and after absorbing and getting excited it emits energy of a particular wavelength(Emmision Spectra).So all kind of strcture determination and chemical properties explanation are related to light used in Spectroscopy.

2)Comparing the wavelength value,we can distinguish different types of Light from high to low wavelength-

radio waves

microwaves

infrared

optical light

ultraviolet

x-rays

gamma rays

3)Spectroscopy has a vast application in Chemical analysis.It has also immense usage in medical sciences.X-ray for bone imaging,UV rays for water purifying,Gamma rays for cancer cell treatment and many more uasges can be found in day to day life.

4)The relationship between Transmittance and Absorbance is given below-

A=log10(1/T)

A=Absorbance and T=Transmittance and they are inversely proportional to each other as absorbance increases, transmittance decreases.

5)The relationship between concentration and Absorbance can be Explained by Beer-Lambert law-

A=ecl

Where A=Absorbance, e=Molar extinction coefficient, l=length of the tube used for measurements.

So, Absorbance and Concentration are linearly variable.

6)If the concentration is increased then the absorbance should also increased but if it is much higher the result can be erroneous as At high concentrations, solute molecules can cause different charge distribution on their neighboring species in the solution. Since UV-visible absorption is an electronic phenomenon, high concentrations would possibly result in a shift in the absorption wavelength of the solution.Also high concentration can affect the refractive indexof the solution resulting deviation from actual result.

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