M18. http://www.systembio.com/downloads/PureFection_prod_sheet.pdf Briefly descr
ID: 57030 • Letter: M
Question
M18.
http://www.systembio.com/downloads/PureFection_prod_sheet.pdf
Briefly describe this products workings and uses, and claimed advantages.
The product brochure says:
“The PureFection reagent self-assembles nanoparticles in the presence of DNA and RNA. These complexes are readily taken up by target cells for efficient gene delivery.”
Some speculation on your part (or find something in the literature)… Why are such self-assembled complexes (more?) readily taken up by cells than just naked RNA or DNA? I’m wondering.
Explanation / Answer
The naked DNA or RNA show poor cellular uptake and are instable under physiological conditions. Immunogenicity plays an important role in decreasing the efficiency and uptake of naked DNA and RNA. The naked DNA or RNA that has entered the cell by calcium-phosphate method remains in the cytoplasm and cannot enter the nucleus. Thus, the entered DNA or RNA has no importance.
The self-assembled nanoparticles increase their stability under physiological conditions. These complexes also help in efficient delivery of nucleic acids to the target site. The self-assembled nanoparticles cause temporary destabilization of cell membrane. Hence, self-assembled nanoparticles are readily taken up by cells rather than naked DNA or RNA.
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