A novel octapeptide is isolated from the skin of a poisonous frog. The biotechno

Question

A novel octapeptide is isolated from the skin of a poisonous frog. The biotechnology firm you work for gives you the following data and asks you to determine the sequence so that larger quantities can be made by peptide synthesis and used as an insecticide. For full credit. Explain the function of each reagent, enzyme, procedure and the results at each of steps 1-7, Be thorough and explain your reasoning. (Each part is 2 of 18 pts. and I want something written for each point even if obvious) The amino acid composition following: 1. treatment with DTT and 2. acid hydrolysis of 1 nmole of the peptide gives the following results. What is your interpretation of this data? and what did Step 1 and 2 do? Estimate the MW of the octapeptide ftp the nearest 100) How did you get this number?

Explanation / Answer

The octapeptide is made up of alanine (A), serine (S), lysine (K), phenylalanine (F), valine (V), glutamic acid (D), proline (P), and cysteine (C). The molecular weight of amino acids in the octapeptide is 89, 105, 146, 165, 117, 147, 115, and 121 Daltons respectively.

The molecular weight of proteins is calculated as follows:

The water molecular weight is 18.015 Daltons.

Substitute the values in above equation.

Hence, the molecular weight of the given octapeptide is 879 Daltons.

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