the class im taking is called instrumental analysis... You want to determine the
ID: 570912 • Letter: T
Question
the class im taking is called instrumental analysis... You want to determine the concentration of the amino acid below in the mushroom. So you took 5 g of mushroom and crushed with a mortar and pestle; then allowed to sit in 25 mL of methanol to extract the amino acids. You filtered it and have a methanolic sample solution to test.
A liquid chromatography experiment determined that the concentration of amino acid in the methanolic solution was 0.90 mg/L. What is the concentration of amino acid in the mushroom?
Explanation / Answer
amino acid in the methanolic solution = 0.90 mg/L.
amino acid in 25 mL of methanolic solution = (0.90/1000) x 25 = 0.0225 mg
amino acid in 5 g of mushroom = 0.0225 mg
The concentration of amino acid in the mushroom= 0.0045mg/ gram
= 4.5 mg/kg
= 4.5 ppm
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