1. Consider that in the medium described in the previous question that there wer

Question

1. Consider that in the medium described in the previous question that there were 2 carbon sources, glucose and lactose. The generation times with glucose and lactose are 25 and 30 minutes, respectively. The medium is inoculated with 5 E. coli cells which initially only use the glucose. After one hour of growth, the glucose being exhausted, the bacteria use the lactose. How many cells total would there be after 3 hours of growth?

2.There are initially 2 E. coli bacteria in 200 grams of hamburger meat. Health Canada considers that a load equal to or higher than 20 bacteria per 100 grams of meat represents a risk for the health of consumers. If the growth rate of E.coli at 4oC (mean temperature of a refrigerator) is 0.01 cell/min. what would be the maximum amount of time that the meat could be stored in the fridge before it is non comestible?

Explanation / Answer

50 cells

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