Q8: Why must the level of the solution in the developing chamber be below the sp
ID: 575107 • Letter: Q
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Q8: Why must the level of the solution in the developing chamber be below the spot on your TLC plate?
Q9: Consider the following compounds and the solubility table below. Recall that CH2Cl2 is the same as DCM (methylene chloride; dichlormethane).
How could a mixture containing three (3) compounds, A, B, and C be separated using a separatory funnel more than once? Explain your answer by drawing the separatory funnel(s) and properly labeling layer(s) and position(s) of the compounds.
Hint: Refer to the Evans' pKa Table (linked from the References module) for relative pKa values for these compounds.
Question 8 1 pts Why must the level of the solution in the developing chamber be below the spot on your TLC plate? HTML Editor - ParagrapExplanation / Answer
8. If the level of the solution (eluent) is more than that of spots, then the compound that is present in the spot will dissolve and prevent it to run on to the TLC plate. If the spot is not running on to the TLC plate, the isolation of a mixture of compounds corresponding to the spot can't be decided by using that particular eluent.
Hence to decide the isolation of the mixture of compounds corresponding to a spot, the level of the solution in the developing chamber must be below the spot on your TLC plate.
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