) Based on the partial amino acid sequence provided, do you think that it is mor
ID: 57592 • Letter: #
Question
) Based on the partial amino acid sequence provided, do you think that it is more likely that this region of p77 associates with a cellular membrane or is exposed to the cytosol? A) Why or why not? B) Describe how you would experimentally demonstrate if p77 was associated with the membrane or the cytosol. 1
1YFNPT 6DSWPT 11LAPAN 16YSFLF 21FLARY 26WYINL30
Tyrosine-Phenylalanine-Asparagine-Proline-Threonine- -Aspartate- Serine-Tryptophan-Proline-Threonine- -Leucine-Alanine-Proline-Alanine-Asparagine- -Tyrosine-Serine-Phenylalanine-Leucine-Phenylalanine -Phenylalanine-Leucine-Alanine-Arginine-Tyrosine -Tryptophan-Tyrosine-Isoleucine-Asparagine-Leucine-
Explanation / Answer
The given peptide sequence is not exposed to cytosol because it is not rich in alanine and arginine, It has polar amino acids in relatively abundance hence it is could be extracellular.
It can be experimentally demonstrated by analyzing the structure in different environment.
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