please answer Compound S is a chemical that can form covalent bonds with free su
ID: 57815 • Letter: P
Question
please answerCompound S is a chemical that can form covalent bonds with free sulfhydryl (-SH) groups of cysteine side gains. The reaction can be summarized as follows where "R" indicates the remainder of the cysteine side gain and protein single Compound S molecule can form a single covalent bond with one sulhydryl sulfur atom at a time. The graph below shows the results of an experiment where Compound S was added to a protein sample before and after that sample was subjected to conditions that cause reducation of any disulfide bonds normally present in the natively folded, unreduced protein The reducing conditions were varied in strength and assigned different levels, ranging from 0 (non-reducing, NR) to IV (most reducing) Which statement best supports the results obtained in this experiment? The protein most likely contains eight cysteine side chains, four of which form disulfide bonds in the natively folded, non-reduced state The protein most likely contains four cysteine side chains, an of which form disulfide bonds in the natively folded, non-reduced state The protein most likely contains four cysteine side chains, three of which form disulfide bonds in the natively folded, non-reduced state The protein most likely contains eight cysteine side chains, six of which form disulfide bonds n the natively folded non-reduced state The protein most likely contains eight cysteine side chains, two of which form disulfide bonds in the natively folded non-reduced state
Explanation / Answer
Answer: the protein most likely conatins eight cysteine side chains, six of which form disulphide bond in the natively folded, non reduced state
Justification: Reducing conditions is state at which S-S bond is reduced to S-H bond. The graph depicts that at the reducing condition III the total number of compond S molecule bound per protein remains is 8 and even further increase in condition resulted in constant output 8, this signifies that the total number of compond S bounded to protein is 8. Therefore at the intial when there was no reducing condition, then also 2 molecules of compound are observed, this signifies that still six of the molecules are remain bonded to the protein which are released during the reducing condtition only when S-S is cleaved to S-H
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