(a) The piece of prokaryotic DNA shown below has a promoter for a housekeeping g
ID: 57945 • Letter: #
Question
(a) The piece of prokaryotic DNA shown below has a promoter for a housekeeping gene that is recognized by 70. Circle and name the two consensus regions of the promoter of the prokaryotic gene. (Hint: The underlined A in the coding strand is the transcription start site. Be sure to
circle the correct strand.)
b) Transcribe the RNA that would be transcribed by E.coli RNA polymerase from the dsDNA shown below. Be sure to indicate the 5’ and the 3’ end of the transcript.
Coding strand 5’GCTGGGTTGACACCTACTCAAACGGCTTATAATGATTGCAGCTACAT...3’ 3’CGACCCAACTGTGGATGAGTTTGCCGAATATTACTAACGTCGACTTA...5’ Template strand
Explanation / Answer
b)
The transcribed RNA will be as follows:
5’GCUGGGUUGACACCUACUCAAACGGCUUAUAAUGAUUGCAGCUACAU...3’
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