Is this an actual question? My professor gave me a piece of paper that said ther
ID: 57964 • Letter: I
Question
Is this an actual question?
My professor gave me a piece of paper that said there are 100 fields of 200 red blood cells, which contains a total of 20,000 RBCs.
Then he told me to figure out the total number of RBC in 1 cc or 1 mL.
I know the answer is 5 million (I'm pretty sure?), but what I don't get is...how was I supposed to figure out how many RBCs are in 1 mL or cc with what he gave me? Wouldn't I have to know how many how many CCs or mLs are in a field? How am I supposed to figure out how many mLs of blood are in a field? Isn't every field different depending on how zoomed in you are and what kind of microscope you're using?
Explanation / Answer
Your question is still not clear.
Counts wont change whatever zooming you are using and whatever microscope you are using. Because finally you are counting total number of ceels in all squares by moving the slide.
Here I am explaining how to findout RBC per ml of sample
your professor must have used hemocytometer(slide) to count the red blood cells.which contains 9 squares and again each large square contains 16 large squares except centre square which contains 25 large squares.
A small drop of blood will be added on to the slide and then covered with acoverslip. Then focussed under 40X or 100 X. number of cells in all squares are counted and then average is taken.
The length and bredth of each large square is 1 mm and height (distance between slide and coverslip is 0.1 mm).
So 1 large square = 1 mm × 1 mm × 0.1 mm (length × breadth × height)
= 0.1
So volume above each large square = 0.1 mm3
To get per 1 mm3
= Average count × 10
To get per 1 cm3 (cells per ml)
= Average count × 10000 Gives RBC per ml of sample
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