Because the H PO and HPO system resists large changes ion pH when acid or base i

Question

Because the H PO and HPO system resists large changes ion pH when acid or base is added, it is called a buffer The phosphate buffer system described previously maintains the pH within cells in your body. Calculate the ratio of hydrogen phosphate to dihydrogen phosphate (A/HA) in a cell with an intracellular pH of 7.4 8. 9. Consider the following diagram. What happens to the pH when a significant amount of base is added? Propose a reasonable explanation for this observation Buffer- a. Base Buffer Capacity Capaty Acid Buffer Volume Base Added (-Voluae Acid Added

Explanation / Answer

Q8

apply:

pH = pKa + log(A-/HA)

substitute known data

pKa = 7.21

pH = 7.40

then

7.4 =7.21 + log(a-/HA)

A-/HA = 10^(7.4-7.21) = 1.54881

A-/HA = 1.54881

Q9

if some base is added, then

pH must increase... util the "buffer" is destroyed, and the pH will increase similar to addition to water, that is, unbuffered

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