title of experiment: conducting an experimental investigationin biology: respons
ID: 5821 • Letter: T
Question
title of experiment: conducting an experimental investigationin biology: responses of plant cells to different types ofsolutionsthese are the procedures of the experiment:
1. Bend a piece of Rhoeo discolor leaf, lower surface up,between your thumb and forefinger. With forceps, strip a portion ofthe lower epidermis.
2. Mount the epidermis in a drop of water, cover with a coverslip, and observe under LPO and HPO.
3. Identify individual cells and take note of the followingvisible structures: cytoplasm, cell wall, stoma, guard cells,nucleus.
4. Sketch to show 2-3 pairs of guard cells and surroundingpurplish hexagonal epidermal cells. Take note of the position ofthe cytoplasm with respect to the cell walls. DO NOT ALLOW YOURMOUNT TO DRY UP! (Note the hexagonal cells. The pairs of crescent -shaped cells are guard cells of the stomatal pore. When the leaf isactively photosynthesizing and water is abundant, the guard cellsare swollen or turgid, and the stoma is consequently open. Whenphotosynthesis and water become limiting, guard cells collapse,thus closing the stoma.)
5. Now, add 2 - 3 drops of 40% NaCl to one edge of the coverslip. Touch a piece of tissue paper to the fluid under the oppositeedge of the cover slip. This will wick the salt solution under thecover slip.
6. Observe the cells under LPO and HPO. Observe what happenedto the guard cells and surrounding epidermal cells under bothhypotonic and hypertonic solutions. Note that the cytoplasm isno longer pressed against the cell wall. This shrinkage is calledplasmolysis. Take note also of the displacement of the pigment onone side of the mount.
materials: microscopes, hypotonic solution (distilled water),hypertonic (40%) salt solution, Rhoeo discolor leaves, forceps,blade, glass slides and cover slips, droppers, rubber bulbs. title of experiment: conducting an experimental investigationin biology: responses of plant cells to different types ofsolutions these are the procedures of the experiment: 1. Bend a piece of Rhoeo discolor leaf, lower surface up,between your thumb and forefinger. With forceps, strip a portion ofthe lower epidermis. 2. Mount the epidermis in a drop of water, cover with a coverslip, and observe under LPO and HPO. 3. Identify individual cells and take note of the followingvisible structures: cytoplasm, cell wall, stoma, guard cells,nucleus. 4. Sketch to show 2-3 pairs of guard cells and surroundingpurplish hexagonal epidermal cells. Take note of the position ofthe cytoplasm with respect to the cell walls. DO NOT ALLOW YOURMOUNT TO DRY UP! (Note the hexagonal cells. The pairs of crescent -shaped cells are guard cells of the stomatal pore. When the leaf isactively photosynthesizing and water is abundant, the guard cellsare swollen or turgid, and the stoma is consequently open. Whenphotosynthesis and water become limiting, guard cells collapse,thus closing the stoma.) 5. Now, add 2 - 3 drops of 40% NaCl to one edge of the coverslip. Touch a piece of tissue paper to the fluid under the oppositeedge of the cover slip. This will wick the salt solution under thecover slip. 6. Observe the cells under LPO and HPO. Observe what happenedto the guard cells and surrounding epidermal cells under bothhypotonic and hypertonic solutions. Note that the cytoplasm isno longer pressed against the cell wall. This shrinkage is calledplasmolysis. Take note also of the displacement of the pigment onone side of the mount.
materials: microscopes, hypotonic solution (distilled water),hypertonic (40%) salt solution, Rhoeo discolor leaves, forceps,blade, glass slides and cover slips, droppers, rubber bulbs. materials: microscopes, hypotonic solution (distilled water),hypertonic (40%) salt solution, Rhoeo discolor leaves, forceps,blade, glass slides and cover slips, droppers, rubber bulbs.
Explanation / Answer
The cells in the Rhoeo discolor leaves will plasmolysis whenplaced in a hypertonic solution.
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