The data in the table at the right show a calibration curve for the determinatio
ID: 587398 • Letter: T
Question
The data in the table at the right show a calibration curve for the determination of RNA from its absorbance at 260 nm. Use a spreadsheet and the least-squares method to determine the slope and intercept of the best straight line fit to the calibration curve. (Do not subtract the blank reading when creating your calibration curve.)
Slope =
y-intercept =
Using the slope and intercept of the calibration curve, determine the quantity of RNA in a sample that gives an absorbance of 0.741.
Mass RNA=
RNA Absorbance 0 0.220 11.20 0.578 22.40 0.886 33.60 1.172 44.80 1.442Explanation / Answer
a plot with Absorbance (A) on y-axis and RNA quantity n Y-axis is created.
the plot is shown below.
from the plot of equation of best fit. slope = 0.027( coefficient of x in the equation )
y intercept is obatined by putting x=0 in the equation hence y= 0.027x+0.252
when x=0, y=0.252
when A=0.741= 0.027x+0.252
x= (0.741-0.252)/0.027=18.11
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