ost-Lab Questions L. The Procedure calls fior rigrnues grinding of the onion cel

Question

ost-Lab Questions L. The Procedure calls fior rigrnues grinding of the onion cells with sand. Would you be able to isolabe the DNA in the cells if this step were omited? What is the purpcse of the grinding process? 2 In the experiment, how did you minimize the interaction between the DNA and histones (proteins 3. What compounds remain in the ethanol solution after the DNA is reemoved by spooling onto a glass rod? 4. Could you do without using the detergent in the procedure? Explain your answer 5. Is the diphenylamine reagent able to distinguish between ribose and deoxyribose? Could it also distinguish between DNA and RNA? 6. What is the structural difference between glucose and ribose? Did the diphenylamine test work for the glucose solution? 523

Explanation / Answer

1. You wouldn't be able to isolate DNA in the cells if the grinding step were omitted.

The purpose of grinding process is to extract the DNA from the onion cells: The low starch content of DNA allows the DNA to be seen more clearly.

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