To determine the reproducibility of mutation frequency measurements, you do the

ID: 58947 • Letter: T

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To determine the reproducibility of mutation frequency measurements, you do the following experiment. You inoculate each of 10 cultures with a single E. coli bacterium, allow the cultures to grow until each contains 106 cells, and then measure the number of cells in each culture that carry a mutation in your gene of interest. You were so surprised by the initial results that you repeated the experiment to confirm them. Both sets of results display the same extreme variability, as shown in the table below. Assuming that the rate of mutation is constant, why do you suppose there is so much variation in the frequencies of mutant cells in different cultures?

Culture (mutant cells /106 cells) 7 2 6 Experiment 3 4 5 8 9 10 0 257 32 0 1 4 2 6 2 2 128

Explanation / Answer

There seems to be transposons present in the bacterial genome. That is why there is so much variation in the rate of mutation.

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To determine the relationship between absorbance versus concentration of crystal

To determine the reproducibility of mutation frequency measurements, you do the

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To determine the reproducibility of mutation frequency measurements, you do the

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