Page 3 Laboratory 8 12. The reagent in the SDS PAGE sample buffer that fully red
ID: 591907 • Letter: P
Question
Page 3 Laboratory 8 12. The reagent in the SDS PAGE sample buffer that fully reduces the protein samples is: A) Sodium dodecyl sulfate (SDS) B) Acrylamide C) Bis acrylamide crosslinker D) Beta-mercaptoethanol E) Blue dye 13. The reagent in the SDS PAGE sample buffer that fully denatures the protein samples is: A) Sodium dodecyl sulfate (SDS) B) Acrylamide C) Bis acrylamide crosslinker D) Beta-mercaptoethanol E) Blue dye 14. The reagent in the SDS PAGE sample buffer that shows the lower boundary of the sample as it moves down the gel is: A) Sodium dodecyl sulfate (SDS) B) Acrylamide C) Bis acrylamide crosslinker D) Beta-mercaptoethanol E) Blue dye 15 Briefly describe two important functions of SDS PAGE, and a brief discussion of how these are accomplished. 16. A graph obtained for an SDsS-PAGE experiment of log Mr versus distance the band traveled is shown in the figure below. If HRP treated under r identical conditions traveled 4.5 cm, what is the oyorwtk 3.00 2.50 2.00 1.50 . 1.00 0.50 0.00 10 Distance (cm)Explanation / Answer
Ans 12 E) Blue dye.
The bromophenol blue dye in the SDS - PAGE is used in tracking to monitor the sample during the electrophoretic run. Different staining methods are used to detect the rare proteins present in the sample.
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