uo this LlIS or lil. (No extra for doing both) A biochemist obtains the followin
ID: 592666 • Letter: U
Question
uo this LlIS or lil. (No extra for doing both) A biochemist obtains the following set of data for an enzyme that is known to follow Michaeis Menten kinetics. Substrate concentration (mM) Initial Rate (mM/s) 50 100 1,000 5,000 4.9 9.6 34.9 62.1 67.6 69.8 69.9 (a) Vau: or the enzymeis4 Explain in one sentence how you determined Vo. Explain in one sentence how you determined Vma (b) Km for the enzyme isExplain in one sentence how you determined K se Mn te the Initial velocity based on your estimation of Km and Vmax when [(sl-15 mM. (d) if the concentration of the enzyme in this experiment is S0 nM, what is the turnover number of the enzyme?Explanation / Answer
From the above show enzymatic reaction data,
(a) Vmax for the enzyme = 69.9 mM/s
Vmax is the maximum velocity rate attained by the enymatic reaction which from the data is at 69.9 mM/s.
(b) Km for the enzyme is 8 mM
Km is the substrate concentration at 1/2 of Vmax velocity for the reaction.
(c) using,
V = Vmax[S]/(Km + [S])
with,
[S] = 15 mM,
initial veocity (V) = 69.9 x 15/(8 + 15)
= 45.59 mM/s
(d) when concentration of enzyme [E] = 50 nM/10^6 = 0.00005 mM
turnover number = Vmax/[E]
= 69.9 mM/s/0.00005 mM
= 1398000 s-1
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