Expert Q&A; Done Name: Mystery Lab-Part 3: Chromatography Tests Tour instructor

Question

Expert Q&A; Done Name: Mystery Lab-Part 3: Chromatography Tests Tour instructor may ask you to answer these in your lab notebook, or to answer direct on this page and turn it in, or to include these answers in a formal lab report. Follow you instructor's directions 1) What was the stationary phase? 2) What was the mobile phase? 3) Why did the colors separate? 4) Why would you cover your chromatography chamber? 5) Where should the solvent level be with respect to the dots? 6) Is it better to have a tall chamber or a short one? Why? 7) Why was one solvent better than another?

Explanation / Answer

Chromatography is the technique used to separate the components present in a mixture.Main methods of Chromatography are Column chromatography,Paper chromatography,Thin layer chromatography (TLC) ,Gas chromatography ,Ion exchange chromatography and  high-performance liquid chromatography (HPLC).

1. The phase used in chromatography on which components are adsored is known as Stationary phase. Generally these are solids or liquids. Eg: Silica gel.

2. The phase with which components moves or travells on stationary phase is called as Mobile phase. Generally these are gases or liquids. Eg: Hexane,Ethanol,Argon etc.

3.The colors represents different components which having different partition coefficients. Based on these partition coefficients the components moves with mobile phase on stationary phase with different velocities. Thus the colors will separated.Eg: TLC

4.If we didn't cover the chromaographic chamber then the organic solvents will evaporated fastly and errors occured in measuring Rf values. Eg:Ether will evaporates faster than water hence we have to cover it.

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