Your ki: Your name: Biochemists have discovered more than 400 mutant varieties o
ID: 593255 • Letter: Y
Question
Your ki: Your name: Biochemists have discovered more than 400 mutant varieties of hemoglobin, the blood protein that carries O2. A sample 45.22 mg of the protein was dissolved in water at 15.0 C to make 2.46 mL of solution. This blood protein has a molar mass of 7.02x10 g/mol. What is the osmotic pressure? (10 pts) 6. Ith, «vlnnlof solvat ph-ride fva What is osmosis? Why the osmotic pressure occurs? ,LA,usthe9pplinlo | 'pnscurvet prevost he het flow of solvht Gran of the protein: 422 sans e,045 lors Gram of the protein: 45.22 mg -1 04Swg Molar mass of the 7.02x10' g/mol protein Ideal gas constant, R: Hint: the unit is atm L/mol K Moles of the protein Liters of solution 2.46 ml Absolute temperature: (convert °C to Kelvin) Osmotic pressure in atm = MRT Osmotic pressure in Torr: (1 atm = 760 mmHg) Osmotic pressure in psi: (1 atm = 14.7 psi)Explanation / Answer
Osmosis: The movement of solvent molecules from dilute solution to higher concentration solution through semipermeable membrane is called osmosis.
Osmotic pressure: The extra pressure that must be applied on solution side to just stop osmosis is called osmotic pressure.
Mass of protein = 45.22 mg = 0.04522 g.
Molar mass protein = 7.02 * 104 g/mol
Moles of protein = mass / molar mass = 0.04522 / (7.02*104) = 6.44 * 10-7 mol
Ideal gas constant = 0.0821 L.atm.K-1.mol-1
Liters of solution = 2.46/1000 = 0.00246 L
Molarity = moles / volume = 6.44 * 10-7 / 0.00246 = 2.62 * 10-4 M
Absolute temperature = 15.0 + 273.15 = 288.15 K
Osmotic pressure = M R T = 2.62 * 10-4 * 0.0821 * 288.15 = 6.20 * 10-3 atm
(OR)
Osmotic pressure = 6.20 * 10-3 * 760 = 4.71 mmHg
(OR)
Osmotic pressure = 6.20 * 10-3 * 14.7 = 9.11 * 10-2 psi
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