1. What is the importance of micropipettes and gel electrophoresis in genetic en

Question

1. What is the importance of micropipettes and gel electrophoresis in genetic engineering?

2. During the labs, you were often reminded to avoid contact with the pipette tips—for example, you were asked to put the pipette tip on without using your hands, to avoid setting down the micropipette, to use the ejector button to remove the tip, and to keep the tip box closed. If you were working with plasmids and bacterial cells, why would these precautions be important?

3. Study your gel electrophoresis results:

Which solution sample contained a single dye: S1, S2, or S3? How do you know?

The molecular weights for the dyes are 452.38 atomic units (au) for orange G, 669.98 au for bromophenol blue, and 538.62 au for xylene cyanole. How do these weights compare with your original conclusions about the weights of the dyes?

4. Do you think there was a difference in the amount of charge on one of the dye molecules? Explain the reasoning for your response.

Explanation / Answer

micropipettes aid in pipetting very small amounts of the sample ranging from 0.1 microliters to 1ml. They do the same process with great accuracy and ease.

Gel electrophoresis is a powerful tool in genetic engineering. With its help, the size of DNA and protein samples of variable lengths can be determined. Macromolecules can be separated on the basis of the charge that they carry.

Related Questions

Navigate

• Browse (All)
• Browse 1
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.