You are interested in bacterial cell cycle control and you have noticed that one

Question

You are interested in bacterial cell cycle control and you have noticed that one particular strain of bacteria can adjust its cell cycle based on temperature in a much more sensitive way than most other bacteria you have studied. You have isolated a protein that you think is responsible for this effect, as well as a small molecule (called Q) that binds to this protein. To try and understand how this protein is involved in thermal sensing, you run the following thermal melt on a sample of the purified protein in the presence and absence of ligand Q. a) Derive an expression that explains what effect ligand Q has on the protein, assuming that the ligand can only bind to the native state of the protein.

Explanation / Answer

The ligand Q has an exponential effect on the unfolding of the ligand . So the expression can be given as. Fraction Unfolded = e^x where x is the amount of Q ligand used.

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