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1.if a scientist performed the lipase gelatinase and mannitol test on a species

ID: 60233 • Letter: 1

Question

1.if a scientist performed the lipase gelatinase and mannitol test on a species of bacteria that she is trying to identify, she is most probably trying to distinguish between which two bacterial species? 2.how do bacteria metabolize molecules too large to enter the cell? 3.why are organisms usually either MR or VP positive,not both? 1.if a scientist performed the lipase gelatinase and mannitol test on a species of bacteria that she is trying to identify, she is most probably trying to distinguish between which two bacterial species? 2.how do bacteria metabolize molecules too large to enter the cell? 3.why are organisms usually either MR or VP positive,not both? 2.how do bacteria metabolize molecules too large to enter the cell? 3.why are organisms usually either MR or VP positive,not both?

Explanation / Answer

1. The tests are performed to differentiate between pathogenic and non-pathogenic Staphylococcus species.

Staph. aureus is positive for gelatinease, Staph. epidermidis is negative for lipase enzyme.

Both the species grow in Mannitol medium, but Staph. auresus turns the medium yellow. Staph. epidermidis does not change the medium color.

2. They secrete extracellular enzymes to degrade macromolecules. The enzymes digest the macromolecules outside the cell, they are then absorbed into the cells.

3. The test is done to determine either of the two biosynthetic pathways using pyruvate as substrate. Organisms can either form butanediol through mixed acid fermentation, or can form acetoin through butylene-glycol, but not both at a time.

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