Below is a diagnostic agarose DNA gel in which samples were digested separately
ID: 60251 • Letter: B
Question
Below is a diagnostic agarose DNA gel in which samples were digested separately with two different restriction enzymes and ran in separate lanes. Underlined words represent individual lanes on the gel. Blue boxes represent a band that has migrated in the gel from the top to the bottom. Each band is comprised of DNA fragments that are all the same size. All of the bands with white boxes require you to enter the size of the DNA fragments in base pairs in the box. Note that not all of the bands require you to do this.
For each of the colonies you tested, decide if you would use or if you wouldn't use the plasmid (prior to digestion) isolated from the colony to generate maize plants that express human lysozyme. Then select the best reason why you did or did not decide to use the colony for future experiments. The survival of your company depends on it.
A. No, I would not use this colony.
B. Yes, I would use this colony.
A. the insert was not ligated into the the vector.
B. the insert was ligated into the vector in the non-native orientation.
C. the insert was ligated into the vector in the native orientation.
D. the colony did not contain the recombinant plasmid or the original vector.
Yes, I would use this colony.
No, I would not use this colony.
A. the insert was ligated into the vector in the non-native orientation.
B. the insert was not ligated into the the vector.
C. the colony did not contain the recombinant plasmid or the original vector.
D. the insert was ligated into the vector in the native orientation.
Yes, I would use this colony.
No, I would not use this colony.
A. the colony did not contain the recombinant plasmid or the original vector.
B. the insert was ligated into the vector in the non-native orientation.
C. the insert was ligated into the vector in the native orientation.
D. the insert was not ligated into the the vector.
Yes, I would use this colony.
No, I would not use this colony.
the insert was ligated into the vector in the non-native orientation.
the insert was ligated into the vector in the native orientation.
the insert was not ligated into the the vector.
the colony did not contain the recombinant plasmid or the original vector.
Digested with NcoI Digested with KpnI pBNJ Colony1 Colony2 Colony3 Colony4 pBNJ Colony1 Colony2 Colony3 Colony4 Answer Answer Answer Answer Answer Answer Answer Answer AnswerExplanation / Answer
1a) Colony 1:
B. Yes, I would use this colony.
1b) Because the diagnostic gel demonstrated that...
A. the insert was not ligated into the vector.
2a) Colony 2:
Yes, I would not use this colony.
2b) Because the diagnostic gel demonstrated that...
D. the insert was ligated into the vector in the native orientation.
3a) Colony 3:
No, I would not use this colony.
3b) Because the diagnostic gel demonstrated that...
A. the colony did not contain the recombinant plasmid or the original vector.
4a) Colony 4:
Yes, I would use this colony.
4b) Because the diagnostic gel demonstrated that...
A. the insert was ligated into the vector in the non-native orientation.
1a) Colony 1:
B. Yes, I would use this colony.
1b) Because the diagnostic gel demonstrated that...
A. the insert was not ligated into the vector.
2a) Colony 2:
Yes, I would not use this colony.
2b) Because the diagnostic gel demonstrated that...
D. the insert was ligated into the vector in the native orientation.
3a) Colony 3:
No, I would not use this colony.
3b) Because the diagnostic gel demonstrated that...
A. the colony did not contain the recombinant plasmid or the original vector.
4a) Colony 4:
Yes, I would use this colony.
4b) Because the diagnostic gel demonstrated that...
A. the insert was ligated into the vector in the non-native orientation.
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