1.Which of the following probes will hybridize to this target sequence of DNA? 5

Question

1.Which of the following probes will hybridize to this target sequence of DNA?

5' - AGG TGA CCG TTA ACG CCT - 3'

5' - AGG CGT TAA CGG TCA CCT - 3'

5' - TCC ACG GGC AAT TGC GGA - 3'

5' - TCC GCA AAT GCC AGT GGA - 3'

5' - UCC UCG CCG TTA ACG CCT -

2.

Messenger RNA (mRNA) has a sequence (5' to 3') which exactly corresponds (with the substitution of U for T) to the sequence of the:

ribosomal RNA (rRNA)

template strand of DNA

transfer RNA (tRNA)

coding strand of DNA

3.

What process can be used to make a DNA probe produce the fluorescence of chemiluminescent signal?

Enzymatic attachment of acridinium esters to terminal ends of the probe

Splicing the gene for beta-lactosidase into the probe

Substitution of biotinylated or fluorescent nucleotides into the probe

Heat denaturation of the probe followed by acid treatment

4.

What term describes the products produced when DNA is digested by restriction endonucleases?

Chimeras

Amplicons

Restriction fragment length polymorphisms

Mossaicisms

5.

Which statement accurately describes the process of fluorescent in situ hybridization (FISH)?

Hybridization is performed directly on intact chromosomes.

Hybridization occurs by attachment to the probe only at the centromere.

Hybridization probes are attached to histones associated with the chromosomes.

Hybridization is performed on DNA extracted from cells.

6.

Which of the following is the most common type of polymorphism?

Singular nucleotide polymorphism (SNP)

Short tandem repeat (STR)

Short repetitive interspersed element (SINES)

Variable number tandem repeat (VNTR)

5' - AGG CGT TAA CGG TCA CCT - 3'

5' - TCC ACG GGC AAT TGC GGA - 3'

5' - TCC GCA AAT GCC AGT GGA - 3'

5' - UCC UCG CCG TTA ACG CCT -

Explanation / Answer

1. a. 5' - AGG CGT TAA CGG TCA CCT - 3'

Probe detects the complementary sequences of specific DNA or RNA. The given is a DNA strand, its complementary strands is, 3' - TCC GCA ATT GCC AGT GGA - 5' (complementary strands runs in 3' to 5' direction).

5' - AGG CGT TAA CGG TCA CCT - 3'

3' - TCC GCA ATT GCC AGT GGA - 5'.

2. d. coding strand of DNA

if the coding strand sequence is GTC, its mRNA sequence is, GUC (U in place of T, remaining is same).

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