Below is a portion of a DNA sequencing gel (Sanger method) for a particular huma

Question

Below is a portion of a DNA sequencing gel (Sanger method) for a particular human disease associated allele. A 15-nucleotide (nt) oligonucleotide was used as the sequencing primer. The bottom of the gel represents the positive-pole during the electrophoresis of the samples. a. What is the DNA sequence of this gene starting from the 3'-end of the sequencing primer (first 10- nts only)? b. For the nucleotide marked with the asterisk, what is the length of this nucleotide? c. For the nucleotide marked with the asterisk, how many ddNTPs are present in this fragment? d. What is unusual about this sequence? e. Which human gene is associated with this unusual feature?

Explanation / Answer

a) 5'-ATCATCAGCAGCAGCAGCAGCAGCAG-3'

b) 14 nucleotides

c) One molecule of ddNTP are present.

d) contains CAG repeats

e)Huntington's disease gene shows CAG repeats

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