Thinking Critically: Evaluating Questions and Answers To sofve the problem below

Question

Thinking Critically: Evaluating Questions and Answers To sofve the problem below, you must write a dear and convincing justification for accepting one of the possibe answer choices as the best or most appropriate and for rejecting each of the other choices as less appropriate. Your reasons for rejecting choaices are just as important as your reasons for choosing the best answer. A blologist prepared a strip of agar (a gelatin-like substance) as shown in the figure below. At porition 11, he placed a concentrated sample of amino acid O (ornithine) and at position 1, an equally concentrated sample of enzyme E (ornithine decarbar/lase). E is known to catalyze the conversion of O to the product P (putresine). You can imagine the aroma this contributes to the decay of animal flesh. The temperature of the two substances and the agar are equal and held constant during the experiment. Assume that O and E can diffuse through the agar and that the factors that affect the rate of diffusion must be considered. At what position would you predict you would likely find P and why? Why would the other positions represent Putresine: P location 4 9 10 11 A. 1.5 B. 4 C.6.5 D. 9

Explanation / Answer

A. 1.5 Most probably this could be the answer. This may happen because the enzyme ornithine decarboxylase has a molecular weight of about 60 kDa and that of ornithine is ~132 daltons. Hence te diffusion of ornithine will be much more faster as compared to the enzyme. Hence it will reach the other end soon and lead to the formation of the product.

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