Need an explanation, not just an answer. If two proteins, one large and one smal

Question

Need an explanation, not just an answer.

If two proteins, one large and one small are added to a gel filtration column the following

happens:

The smaller protein elutes first and the larger protein enters into the pores

The larger protein elutes first and the smaller protein enters into the pores

Elution of the two proteins occurs simultaneously

The more electronegative protein elutes first

The smaller protein elutes first and the larger protein enters into the pores

The larger protein elutes first and the smaller protein enters into the pores

Elution of the two proteins occurs simultaneously

The more electronegative protein elutes first

Explanation / Answer

Based on the given data,

In gel filtration chromatography the larger proteins elutes first from the column and then the elution of smaller proteins takes place. This is because after addition of crude sample, the smaller proteins are trapped inside the gel, but larger proteins cannot. So, in first washing larger proteins elute from column and in second washing smaller proteins will elute.

Thus, the correct option is (b) the larger protein elutes first and the smaller protein enters into the pores.   

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