Which statement best describes the process of western blotting and the use of EC

Question

Which statement best describes the process of western blotting and the use of ECL

A radioactive group is added to an RNA probe which is incubated with a gel containing the

complementary sequence

A secondary antibody is conjugated to horse radish peroxidase which causes

oxidative degradation of luminol and emission of photons

A promoter is fused to luciferase which causes luminescence by using luciferin as a

substrate

A protein is conjugated to GFP and ECL is utilized to quench the valence shell electron to

visualize the protein

A radioactive group is added to an RNA probe which is incubated with a gel containing the

complementary sequence

A secondary antibody is conjugated to horse radish peroxidase which causes

oxidative degradation of luminol and emission of photons

A promoter is fused to luciferase which causes luminescence by using luciferin as a

substrate

A protein is conjugated to GFP and ECL is utilized to quench the valence shell electron to

visualize the protein

Explanation / Answer

A secondary antibody is conjugated to horse radish peroxidase which causes oxidative degradation of luminol and emission of photons

Horse Radish Peroxidase (HRP) conjugated to the secondary antibody catalyzes the oxidation of luminol into a substance which emits light when it decays.

Here HRP conjugated secondary antibody binds to the primary antibody, which inturn is bound to the protein of interest on the membrane.

The amount and location of light that HRP catalyzes the emission of, is directly correlated with the location and amount of protein on the membrane.

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