Using transfection of a mammalian plasmid, how can one achieve a pseudo-100% tra
ID: 61267 • Letter: U
Question
Using transfection of a mammalian plasmid, how can one achieve a pseudo-100% transfection
efficiency?
Use of a plasmid that encodes a protein which degrades a toxic drug and allows only
expressing cells to survive in the presence of a drug
Use of a plasmid that encodes a protein which generates a toxic drug
Use of a plasmid that encodes a protein which generates a toxic protein
Use of a plasmid that encodes a temperature sensitive mutant protein that is degraded at
low temperatures
Use of a plasmid that encodes a protein which degrades a toxic drug and allows only
expressing cells to survive in the presence of a drug
Use of a plasmid that encodes a protein which generates a toxic drug
Use of a plasmid that encodes a protein which generates a toxic protein
Use of a plasmid that encodes a temperature sensitive mutant protein that is degraded at
low temperatures
Explanation / Answer
Answer : Use of a plasmid that encodes a temperature sensitive mutant protein that is degraded at
low temperatures.
It will make sure that the nucleic acid succesfully delivered to the target cell. It is harmless as the nucleic acid is coded by mutant protein which will be degraded at low temperature so it can maintain its 100% pseudo transfection efficiency.
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