Reciprocal crosses were performed using two inbred strains of mice, AKR and PWD,
ID: 61436 • Letter: R
Question
Reciprocal crosses were performed using two inbred strains of mice, AKR and PWD, that have different alleles of many polymorphic loci. In each of the two crosses, placental tissue was isolated whose origin was strictly from the fetus. RNA was prepared from the fetal placental tissue and the subjected to "deep sequencing" (that is, RNA-Seq) Because of the polymorphisms, investigators could compare the number of "reads" of mRNAs for specific genes that were transcribed from maternal or paternal alleles, as shown in the following figure, (The x-axis shows the percentage of reads for the given mRNA that correspond to the AKR allele of that gene.)
C. Using the same type of diagram that indicates the percentage of AKR alleles, diagram the expected results for these same three genes if a female F1 mouse from the cross on the left (that is, a daughter of a cross between an AKR female and a PWD male) was then crossed to a PWD male. Describe the two possible outcomes for each gene.
Gene A Gene B Gene C 50% 0% 50% Percentage of AKR allele 100% 100%Explanation / Answer
The two possible outcomes for each gene are
Gene A 50 or 100
Gene B 100 or 50
Gene C 50 or 100
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