Type the answer to the following questions and turn in QNE WEEK after lab sessio
ID: 61476 • Letter: T
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Type the answer to the following questions and turn in QNE WEEK after lab session, since you have a formal lab report for this lab, this MINI post lab will only count for 10 points. This gel has approximately 10 mug of protein in each lane What would this gel look like if you loaded 1 mu g of protein in each What would this gel look like if you loaded 10 mu g of protein this was run for 90 minutes What would this gel look like if we only ran it for 30 minus what would this gel look like if we ran it for 3 hours protein ladder similar in function . 0 the Protein Standards that we used in the brand AssayExplanation / Answer
1a. wells/lanes
1b. Protein B is more abundant
1c. Size of A is approx 100 KDa
1d. Size of B is approx 36 KDa
2a. The bands appear faint and thin, than they appear now. It will be further easy to resolve them as separate bands, but some of the bands may not be visible
2b. If more sample is loaded, the bands become dense or thicker, they overlap each other, and become fuzzy. It will to difficult to distinguish individual bands
3a. The bands will not separate or resolve if they are electrophoresed for less time period, they will not separate enough to identify as individual bands
3b. Some of the protein bands will migrate off from the gel, and will be lost into the buffer
4. The protein ladder is a molecular weight marker. The proteins present in them are of defined molecular weight and gives a specific resolution or band pattern, where electrophoresed for a specified time period. Just like the concentration of a protein stardard is known to us, the molecular weight of each of the bands is known. By comparing with the standards, the molecular weight of the unknown can be derived.
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