Using the table above, answer the questions on the worksheet. NOTE: The crossed
ID: 61479 • Letter: U
Question
Using the table above, answer the questions on the worksheet. NOTE: The crossed out equilibrium was a typo as shown, it should say Linked: Yes or No.
Initial crosses: Pure breeding wild-type males x Pure breeding black-vestigial-brown females Pure breeding black-vestigial-brown males x Pure breeding wild-type females All progeny from both crosses were wild-type phenotype. Test crosses (class data): F1 virgin females x Pure breeding tri recessive males Males Females Wings vg +vg vg vg vg vg vg + vg + +br b+br+brbbr+br+br 5| 8| 3| 3 2| 2 2 0 9 9 2 9 2 14 812 2 10 24 85 9 7 5 2 3 0 49 3 8 0 3 21 9 5 98 4 2 4 51 15 1985:11 6.9/3:4 19/ 9/ 8 0:5:5 3:2 6 4 8 4 7 2 3 2 2 3 5 26 2 42 3 723 11 9 2 32 2 2 21 23 15 11 6 126 810 16 11 6 223 49 15 10 117 23 2 0 17 19 5 11 6 2 0 10 20 24 12 15 42 2 34 2515 3 11 5 0 11 4 9 2 9 331 12 18 5124 11 13 3 3 2 2208 44 21 0 0 14 5 3 2 3 0 0 00241 3 0 0 0 0 15 8 3 42 1 16 19 13 5 7 5 6 6 2 3 34 2 3 0 3 0 2 30 11 9 9 5116 0 0 9 5 8 4 3 2 2 6 02214 13 12 4 15 5 1814 12 5 9 5 4 3 611 5 7 4 13 9 26 19 134 240 206 3 1 8051 21 22 0 0 0 000 00000 0 0 00 2 883 8 03 0 25 0 00000 00 00 00 0 0 2600 0 000 000 0 0 0 0 00 0 27 0 0 0O0 0 280 0 0 000 00000 0 0 00 214 166 112 100 57 57 56 29 261 178 124 131 70 77 64 30 Total Males-791 Total Females = 935Explanation / Answer
a)
Sex linked
Black (yes)
Brown (yes)
Vestigial (yes)
b)
1 : 1 (wild type : black mutant)
c)
Lets consider male block first
Parental genotype number = 214 + 116
Double cross over recombinant number = 56 + 29
Single cross over recombinants between brown and vestigial = 112 + 100
Single cross over recombinants between vestigial and brown = 57 + 57
Recombination frequency between brown and vestigial = 112 + 100 + 56 + 29 / 791 = 37.5 %
Map distance between brown and vestigial = 37.5 mU
Recombination frequency between vestigial and black = 57 + 57 + 56 + 29 / 791 = 25 %
Map distance between vestigial and black = 25 mU.
Thus map distance between brown and black = 62.5 mU
Likewise for female block, we have
map distance between brown and vestigial = 37.3 mU
map distance between vestigial and black = 25.7 mU
map distance between brown and black = 63 mU
Average map distance between brown and vestigial = 37.4 mU
Average map distance between vestigial and black = 25.35 mU
Average map distance between brown and black = 62.75 = 63 mM
d)
Yes, they are linked
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