1. Isopeptide bonds can form between a-carboxylate groups and a side chain _____

Question

1. Isopeptide bonds can form between a-carboxylate groups and a side chain _____________ group, such as is found in the amino acid _____________ or an a-amino group and a side chain ______________ group such as is found in the amino acids (entered alphabetically) ______________ and _______________.

2. A column containing carboxymethyl groups can be used to seperate serum albumin and ribonuclease A. Serum albumin has a pI of 4.9, so at pH = 7.0 it is (uncharged, positively, or negatively charged?). Ribonuclease A has a pI of 9.4, so it is (uncharged, positively, or negatively charged?). This means that _____________ will bind to the column, since it is a (anion or cation exchange?) column, while ______________ will flow through.

Explanation / Answer

1. Isopeptide bonds can form between a-carboxylate groups and a side chain amino group, such as is found in the amino acid lysine or an a-amino acid group and a side chain carboxyl group such as is found in the amino acids glutamate and aspartate.

2. A column containing carboxymethyl groups can be used to seperate serum albumin and ribonuclease A. Serum albumin has a pI of 4.9, so at pH = 7.0 it is (uncharged, positively, or negatively charged?). Ribonuclease A has a pI of 9.4, so it is (uncharged, positively, or negatively charged?). This means that Ribonuclease A will bind to the column, since it is a cation exchange column, while serum albumin will flow through.

Related Questions

Navigate

• Browse (All)
• Browse 1
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.