All aspects of the Kirby Bauer test are standardized to assure reliability. A. C

Question

All aspects of the Kirby Bauer test are standardized to assure reliability.
A. Consequence of pouring the plates 2 mm deep instead of 4 mm deep?
B. The Mueller-Hinton II plates to be used within a specific time after the preparation and should be free of visible moisture. Why do you think this is so? All aspects of the Kirby Bauer test are standardized to assure reliability.
A. Consequence of pouring the plates 2 mm deep instead of 4 mm deep?
B. The Mueller-Hinton II plates to be used within a specific time after the preparation and should be free of visible moisture. Why do you think this is so? All aspects of the Kirby Bauer test are standardized to assure reliability.
A. Consequence of pouring the plates 2 mm deep instead of 4 mm deep?
B. The Mueller-Hinton II plates to be used within a specific time after the preparation and should be free of visible moisture. Why do you think this is so?

Explanation / Answer

A. The diffusion efficiency of drug can be increased by decreasing the thickness of an agar layer, which results in increased zone of inhibition.

B. Presence of moisture increases the diffusion of drug. So, a false assumption regarding the zone of inhibition may results.

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