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Chose 1 article of scientific journals from the last 3 years (2012-2015) Content

ID: 62153 • Letter: C

Question

Chose 1 article of scientific journals from the last 3 years (2012-2015) Content of the written assignment: 1 . Introduction (relevance of the topic) 2. Background (previous finding on the topic) 3 . Purpose (what the researchers want to do) 4. Results (what the researchers found) 5. Discussion (comment of what the research have done in the paper, use your judgment and opinion) 6. Conclusions Topics: 1 . Iron porphyrin in oxygen transport 2. Copper superoxide dismutase 3. Sulfur clusters in biological systems 4. Molybdenum and Nitrogen fixation 5. Cobalt in biological systems 6. Copper chaperone in SOD 7. Magnesium dependent enzymes 8. Cytochromes 9. Photosystem II and manganese function 10. Photosystem I and copper function 1 1 . Metal centers in catalases 12. Metal centers in methane mono oxygenases

Explanation / Answer

8.

Introduction: Cytochrome P450 hydroxylates lipophilic compounds and the P450 monooxygenase system lacks substrate specificity.

Background: Allan Conney and others described that treatment of animals with different microsomal inducers like phenobarbital and 3-methylcolanthrene, results in different monooxygenase activity for metabolism of xenobiotics and steroid hormones. The species, diet, sex, age of animal are considered that are exposed to a chemical.

Purpose: To prove that there are multiple P450 enzymes with different substrate specificities in single microsomal extract.

Results: P450 catalytic profile alters in different membrane environment and there are multiple P450 enzymes with different substrate specificities.

Discussions: the multiple forms of P450s based on anomalies in P450 spectra were presented by Sato and Manning laboratories.

Conclusion: In two papers, which were published by Jud Coon in 1975 and 1976 confirms the presence of four isoforms of P450. He purified four isoforms of P450 from the rabbit liver.

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