13. Wh en replication of the E.C structure of the dinucleotide? replication of t

Question

13. Wh en replication of the E.C structure of the dinucleotide? replication of the E. coli genome begins, after the first phosphodiester linkage is made, what is the o OO o OO HO-p-0-p-0 HO-p-o-p-o Ho-p-o-CH2 OH O oP-O-CH Base OH OH OH OH OH H OH OH B. 14. What is the structure of the molecule after DNA polymerase II forms its first phosphodiester bond? Only the dinucleotide at the 3'-end is shown. CH Base CH2 Base …CH CH Base 2 Base-CH 2 Base CH 2Base O OH OH oep-o-CH Base -O-CH2 o=p-O-CH2 Ozp-O-CH2 Osp-O-CH2 Base Base Base OH OH OH H OH OH OH H OH H 15. What is the structure of the molecule after DNA polymerase I forms its last phosphodiester bond? Only the dinucleotide at the 3'-end is shown. CH2 Base 2Base CH CH2 BaseCH2 2 Base-CH 2 BaseCH O H O H O OH 00-CH2 0sp-o-CH2 Osp-O-CH2 0 1-0-CH2 Osp-O-CH2 Base Base Base Base Bae OH OH OH H OH OH OH H OH H

Explanation / Answer

13. The correct option is (C).

* Option (A) contains ribose sugars which are not present in DNA

* Option (B) contains ribose sugars, and the bond formed is 5' - 2' bond which is not correct

* Option (D) shows ribose sugars + the newly added nuclotide is shown as Nucleotide monophosphate, but the original participant is nucleotide triphosphate.

Only option (C) shows Deoxy ribose sugars which formed a 3' - 5' phosphodiester bond. Hence, it is the correct answer.

14. The correct answer is option (B).

* Option (A) shows ribose sugars, which are not part of DNA

* Option (C) shows a Deoxy ribose sugar and a ribose sugar; ribose sugar does not exist in DNA

* Option (D) also shows a Deoxy ribose sugar and a ribose sugar; ribose sugar does not exist in DNA

* Option (E) shows a Deoxy ribose sugar and a ribose sugar, and also the linkade between two nucleotides in 5' - 2' linkage, which does not occur normally

15. The correct answer is option (B), the only option which shows deoxy ribose sugars in correct orientation and a 5' - 3' linkage. The other options show either ribose sugars or 5' - 2' linkage, which does not occur naturally in DNA

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