Background for questions 15-17. From the bacterium Staphylococcus aureus you hav

Question

Background for questions 15-17. From the bacterium Staphylococcus aureus you have isolated a gene encoding an antibiotic-degrading protein along with its promoter. You plan to isolate mutants with alterations in the promoter to explore its regulation and interaction with RNA polymerase.

15. What would be the most likely consequence on transcription of the antibiotic-degrading gene of changing all the A-T pairs in the sequence 10-bases upstream from the mRNA transcription start site to G-C pairs?

A. Increase transcription

B. Decrease transcription

C. Would not influence transcription

16. What would be the most likely consequence on transcription of the antibiotic-degrading gene of substituting all the A-T base pairs with the same number of G-C base pairs in the sequence between the -10 and -35 conserved sequences?

A. Increase transcription

B. Decrease transcription

C. Would not influence transcription

17. What would be the most likely consequence on transcription of the antibiotic-degrading gene of deleting five bases pairs in the sequence between the -10 and -35 conserved sequences?

A. Increase transcription

B. Decrease transcription

C. Would not influence transcription

*EXPLANATIONS WOULD BE AWESOME*

Explanation / Answer

15)B

-10 sequence has conserved sequence of TATAAT..its important for interactiin with sigma factor. RNA polymerase

16) c

Sequence between _10 and -35 is not conserved varies from promoter to promoter

17)c

Related Questions

Navigate

• Browse (All)
• Browse B
• Subjects

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.