Based on these results: 1. Give a short description of what you would see for gr

Question

Based on these results: 1. Give a short description of what you would see for growth on the plates if both antibiotic resistances (amp and strep) resided in the genomic DNA instead of the plasmid? 2. What is the drug resistance of strain 1? Strain II? 3. Which plate shows growth in the mating sector that cannot be explained by either strain alone? Assume that one antibiotic resistance gene has been transferred. What are the possible transfers? 4. On which plates do you expect your test colonies to grow? On which plates are you unsure? Are there plates on which you don't expect growth? Why? 5. Describe the donor and recipient strains as well as any gene transfer that has occurred and indicate which observations led you to this conclusion. Sector I Sector I1 Sector III LB (no drug) Table 1. Recorded growth results in each sector Tabl 2. Recorded reslts after amp/strep mating sector cells wene transferred lto test plates

Explanation / Answer

1. If the resistance to drugs are present in the genome and not in the plasmid, they wont be transferred to the other strain. Only genes on the plasmid are transferred. hence, no growth would be seen on the media plates containing antibiotic.

2. Strain 1 is resistant to streptomycin since we see growth on strep plates. Strain 2 is resistant to Amp and Nal.

3. The last plate Amp/Strep in the mating sector shows growth which is not seen in strain 1 or 2. Strep resistance gene has been transferred from strain 1 to strain 2. This can be concluded because the mating sector growth shows more similarity with strain 2.

4. Growth should be seen in all 4 plates. The results seen in the mating sector should be seen in the test plates. The strep/Nal plate would be the plate where growth may not be seen. Since both strain 1 and 2 do not show growth, we can assume that growth may not be seen.

5. Donor is strain 1 and recipeint is strain 2. The gene transferred to strep resistance gene. The mating sector has more in common with strain 2 than strain 1. It shows the properties of strain 2 with the addition of strep resistance.

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