Based on these results 1. Give a short description of what you would see for gro

Question

Based on these results 1. Give a short description of what you would see for growth on the plates if both antibiotic resistances (amp and strep) resided in the genomic DNA instead of the plasmid? 2. What is the drug resistance of strain I? Strain II? 3. Which plate shows growth in the mating sector that cannot be explained by either strain alone? Assume that one antibiotic resistance gene has been transferred. What are the possible transfers? 4. On which plates do you expect your test colonies to grow? On which plates are you unsure? Are there plates on which you don't expect growth? Why? 5. Describe the donor and recipient strains as well as any gene transfer that has occurred and indicate which observations led you to this conclusion.

Explanation / Answer

Str resistant gene is present in strain/sector I and III . Amp resistance gene is present in Sector II and III plates. Nal resistance gene is present in Strain II and III.

In strep/nal plate, growth should have been there in stain III, because strain III is resistant for str, Nal, and Amp (all three).

2.Stain I has resistance for str only. It is sensitive to both Amp and Nal. That is why, it doesn't grow in any other media. Strain II has resistance for Amp and Nal, but is sensitive to Str.

3. Strep/Nal plate; Either the Str or the Nal gene has been transferred. That is why, growth is not seen in any of the three plates.

4. Test colonies are those which has resistance for all three. They will grow on plateIII..

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